磷脂酰類混合物的測定
更新時間:2019-07-08 點(diǎn)擊次數(shù):1192
![](http://img68.chem17.com/9/20190708/636981958360241196755.png)
我們今天做的是磷脂酰類混合物的測定,本次的磷脂酰類混合物主要包括磷脂酰膽堿。
磷脂酰膽堿是細(xì)胞膜和肺部表面活性物質(zhì)的重要成分,在磷脂雙分子層的外葉上更為常見。磷脂酰膽堿轉(zhuǎn)移酶是將磷脂酰膽堿在膜與膜之間運(yùn)輸?shù)妮d體。
磷脂酰膽堿還在細(xì)胞的信號通路中扮演重要的角色。
色譜條件
色譜柱:月旭Ultimate®SiO2(4.6×150mm,5μm);
流動相:
A相:正己烷(含0.04%三乙胺)/異丙醇/13%乙酸溶液=40/57/3;
B相:正己烷(含0.04%三乙胺)/異丙醇/13%乙酸溶液=40/50/10;
時間 | A相 | B相 |
0 | 100 | 0 |
10 | 0 | 100 |
18 | 0 | 100 |
18.1 | 100 | 0 |
25 | 100 | 0 |
檢測器:漂移管線溫度:110℃;載氣壓力:3.0L/min;
柱溫:35℃;
流速:1.5mL/min;
進(jìn)樣量:20μL;
譜圖和數(shù)據(jù)
1、混合對照溶液:
![](http://img69.chem17.com/9/20190708/636981958887351680642.jpg)
![](http://img69.chem17.com/9/20190708/636981959389665295293.jpg)
2、溶血磷脂酰膽堿(LPC):
![](http://img71.chem17.com/9/20190708/636981959548160279944.jpg)
![](http://img70.chem17.com/9/20190708/636981959804466636114.jpg)
3、磷脂酰膽堿(PC):
![](http://img70.chem17.com/9/20190708/636981960019901255894.png)
![](http://img69.chem17.com/9/20190708/636981960192280150121.jpg)
4、磷脂酰絲氨酸(PS):
![](http://img69.chem17.com/9/20190708/636981960407714769250.jpg)
![](http://img71.chem17.com/9/20190708/636981960522530033794.jpg)
5、 溶血磷脂酰乙醇胺(LPE):
![](http://img71.chem17.com/9/20190708/636981960687888973104.png)
![](http://img70.chem17.com/9/20190708/636981960898019626617.jpg)
6、 磷脂酰肌醇(PI):
![](http://img70.chem17.com/9/20190708/636981961100974325810.jpg)
![](http://img71.chem17.com/9/20190708/636981961290825108690.jpg)
7、磷脂酰乙醇胺(PE):
![](http://img70.chem17.com/9/20190708/636981961463048004500.png)
![](http://img68.chem17.com/9/20190708/636981961689714551421.jpg)
8、鞘磷脂(SM):
![](http://img69.chem17.com/9/20190708/636981961844621558107.jpg)
![](http://img71.chem17.com/9/20190708/636981961993132606633.jpg)
結(jié)論
月旭Ultimate®SiO2(4.6×150mm,5μm),在此條件下測定,可以達(dá)到測試要求。